| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Guan, Fiona (10)
|
AMANSC |
44.10 |
40.04 |
-4.06 |
| 2 |
Khanolkar, Mia S (9)
|
AMPTSC |
47.48 |
46.85 |
-0.63 |
| 3 |
Davidson, Sophia R (10)
|
AMANSC |
51.32 |
47.09 |
-4.23 |
| 4 |
Long-Lewis, Emily S (10)
|
AMPEAQ |
55.50 |
51.15 |
-4.35 |
| 5 |
Maddox, Reagan J (10)
|
AMANSC |
53.77 |
52.67 |
-1.10 |
| 6 |
Dwenger, Mia M (9)
|
AMWAAC |
52.74 |
53.03 |
0.29 |
| 7 |
Linneman, Alice E (9)
|
AMPTSC |
1:02.21 |
56.05 |
-6.16 |
| 8 |
Gribble, Elizabeth M (10)
|
AMWHAT |
53.27 |
56.19 |
2.92 |
| 9 |
Miller, Cora J (10)
|
AMANSC |
1:07.77 |
58.73 |
-9.04 |
| 10 |
Brennan, Elizabeth M (10)
|
AMSTEE |
58.31 |
1:00.36 |
2.05 |
| 11 |
Emekter, Selin E (10)
|
AMANSC |
58.95 |
1:04.02 |
5.07 |
| 12 |
Rees, Leia K (9)
|
AMNHA |
1:02.87 |
1:07.21 |
4.34 |
| 13 |
Dabravalskas, Jovi R (10)
|
AMSVSC |
1:23.88 |
1:08.78 |
-15.10 |
| 14 |
Reinhart, Kyra M (10)
|
AMSVSC |
1:20.93 |
1:25.62 |
4.69 |
|
Anderson, Rose L (9)
|
AMSVSC |
1:45.52 |
DQ |
- |